Calibration of hplc in pharma

(1 )Measurement of Flow ml/min

this is measurement by flow meter or Cylinder

this is 0.3 ml, 0.5 ml ,1.0ml, 2.0ml ,3.0 ml

(2) Injector / auto sampler linearity test

Correlation coefficient NLT 0.990

(3) Column Compartment Temperature Test:

15 , 30 45 60

(4) Sample Compartment temperature test

5, 10, 15, 25

(5)Drift and Noise test

Drift  +- 2000 AU/hrs

Noise +- 100 AU

(6) Gradient test

Height (A/B/C) / Height of Full scale peak X 100      Limit 9.0 % – 11.0 %

(7) Pristion

NMY 2.0 %

(8) Detector

NLT 0.9990

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